PubReading [143] - Homology directed correction, a new pathway model for point mutation repair catalyzed by CRISPR‐Cas - B. Sansbury, E. Kmiec et al

Gene correction is often referred to as the gold standard for precise gene editing and while CRISPR‐ Cas systems continue to expand the toolbox for clinically relevant genetic repair, mechanistic hurdles still hinder widespread implementation. One of the most prominent challenges to precise CRISPR‐ directed point mutation repair centers on the prevalence of on‐site mutagenesis, wherein insertions and deletions appear at the targeted site following correction. Here, we introduce a pathway model for Homology Directed Correction, specifically point mutation repair, which enables a foundational analysis of genetic tools and factors influencing precise gene editing. To do this, we modified an in vitro gene editing system which utilizes a cell‐free extract, CRISPR‐Cas RNP and donor DNA template to cata ...