Discovery of a new class of cell‑penetrating peptides by novel phage display platform

DOI: 10.1038/s41598-024-64405-w

Central Idea: Researchers developed a novel phage display platform called NNJA (Novel peptides for intracellular delivery by hijacking two cell systems) that discovers cell-penetrating peptides capable of delivering therapeutic cargo to the cell cytoplasm while avoiding lysosomal degradation. This platform addresses a major challenge in the field of therapeutic delivery.

Key Concepts:

The NNJA Platform:

- Engineered a lysosomal cathepsin substrate within phage protein PIII

- Phage that enter lysosomes are cleaved and eliminated from selection

- Only phage that successfully reach cytoplasm survive selection process

- Results in peptides optimized for cytoplasmic delivery

Novel CPP Characteristics:

- Nine amino acids in length

- L ...