The three-dimensional folding of genes within the nuclear space is integral to understanding gene function and regulation, moving beyond the linear DNA sequence. Dr. Neguembor presents immuno-OligoSTORM, a revolutionary imaging strategy that super-resolves the distribution of nucleosomes within specific genes by simultaneously visualizing DNA and histones.

In this episode, we dive into the technology behind immuno-OligoSTORM and how it integrates with restraint-based and coarse-grained modeling approaches to sync super-resolution imaging data with Hi-C contact frequencies and deconvoluted micrococcal nuclease-sequencing information. This results in Modeling immuno-OligoSTORM, a method that allows for quantitative modeling of genes with nucleosome resolution and reveals information about chromatin accessibility for regulatory factors, like  ...