Note sull'episodio
Scaffolding the very long contigs generated from PacBio and Oxford Nanopore assemblies requires long-range information that extends far beyond the capabilities of conventional mate-pair libraries. While mate-pair sequencing can provide links across genomic distances of several kilobases, it is not practical for connecting contigs separated by hundreds of kilobases or millions of bases. To overcome this limitation, technologies originally developed to study chromosome organization have been adapted for genome assembly.
Together, Hi-C and optical mapping have transformed genome assembly by providing chromosome-scale scaffolding information, bridging the gap between long-read assembly and complete reference-quality genomes. These technologies have been particularly important in generating telomere-to-telomere and near-complete genome assemblies for complex organisms.