In this episode, we welcome Dr. Susanne Rafelski, a leading expert in cellular organization, to explore the complex interplay between gene expression and cellular phenotype. The challenge of understanding how a subset of expressed genes influences cellular phenotype is daunting due to the sheer number of involved molecules, their combinations, and the multitude of cellular behaviors they determine.

Dr. Rafelski's research reduces this complexity by focusing on cellular organization—a critical determinant and indicator of cell behavior. She introduces the WTC-11 hiPSC Single-Cell Image Dataset v1, a remarkable dataset containing over 200,000 live 3D cells across 25 key cellular structures. The scale and quality of this dataset have allowed for the creation of a comprehensive analysis framework to convert raw image data into dimensionally red ...